FGF2 (tuna) growth factor

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Introduction

FGF2 (basic fibroblast growth factor or bFGF) has been instrumental in the maintenance and differentiation of cells, particularly in serum-free workflows. Defined Bioscience’s FGF2 (tuna) growth factor possesses the full native sequence for FGF2 derived from T. albacares, the yellowfin tuna, produced as an animal-free, carrier-free, and highly pure growth factor. Previous research has shown the value of cross-species homologs in cell culture applications, offering opportunities to use this growth factor in improving workflows across human and non-human cell line culture. 

Type: Recombinant tuna protein, E. coli-derived, His-tagged
Purity: >95% by SDS PAGE under reducing conditions
Endotoxin: Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg)
Storage & Stability: Upon arrival store at -20°C to -80°C. Protein is stable for up to 12 months when stored at -20°C to -80°C.
Intended use: For research use only

If you are interested in testing multiple FGF2 variants to improve your workflow, be sure to explore our new FGF2 Screening Kit, offering human, bovine, chicken, and tuna FGF2.

Biological Activity

FGF2 (tuna) has an expected EC50 of less than 2 ng/mL, as determined in-house for all lots by an NIH-3T3 cell proliferation assay.

General Use

Each FGF2 variant included in this kit is supplied as a 1 mg/mL solution in storage buffer that can be incorporated into most standard buffer formulations as needed for downstream use. Dosage testing is recommended for optimal performance.

What is Tuna FGF2? A purified, animal-origin-free growth factor containing the native sequence from Thunnus albacares (yellowfin tuna).

Why use it? Provides a cross-species growth factor option for improving serum-free workflows or aquatic/cultivated seafood research.

How is it expressed? Recombinant expression in E. coli, supplied at 1 mg/mL.

What is its biological activity? EC50 < 2 ng/mL in NIH-3T3 proliferation assays.

What are the storage conditions? Store at -20°C, protected from light, for up to 1 year.