FGF2-G3 (human) growth factor

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Biological Activity

FGF2-G3 has an expected ED50 of less than 1 ng/mL, as determined in-house for all lots by an NIH-3T3 cell proliferation assay.

Key Characteristics

FGF2-G3 differs from the canonical wild-type sequence of FGF2 (UniProt accession P09038) at nine key residues to promote increased thermostability and function in cell culture. As opposed to the 12-hour half-life of the wild-type form at 37°C, FGF2-G3 demonstrates a half-life beyond 21 days with minimal loss of function. FGF2-G3 is required at lower levels than the wild-type form for medium formulations (including at 40 ng/mL for our pluripotency medium, HiDef-B8, compared to 100 ng/mL for the wild-type form). FGF2-G3 is critical for use in cell culture conditions greater than room temperature; and helps to eliminate the need for weekend supplementation and daily medium changes for stem cell culture.

General Use

FGF2-G3 is supplied as a 1 mg/mL solution in storage buffer or in a freeze-dried form that can be reconstituted into most standard buffer formulations as needed for downstream use. FGF2-G3 can be substituted directly for wildtype FGF2 in most applications, with dosage testing recommended for optimal performance. It is recommended to reconstitute powder FGF2-G3 to 1 mg/mL with water before use.

References

1- D. Pavel, D. Bednar, P. Vanacek, L. Balek, L. Eiselleova, V. Stepankova, E. Sebestova, M. Kunova Bosakova,
Z. Konecna, S. Mazurenko. A. Kunka, T. Vanova, K. Zoufalova, R. Chaloupkova, J. Brezovsky, P. Krejci, Z.
Prokop, P. Dvorak, J. Damborsky, Biotechnology and Bioengineering 2018, 115, 850.

Why use FGF2-G3? Thermostable for over 21 days at 37°C versus ~12 hours for wild-type, reducing the need for media changes.

How is it supplied? Liquid or lyophilized, at 1 mg/mL.

Can it replace wild-type FGF2? Yes, though optimal dosage testing is recommended.