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Defined Bioscience

Organoid Technology

Organoid Technology Using Human Induced Pluripotent STEM Cells (iPSCS)

Organoids derived from human induced pluripotent stem cells (iPSCs) are 3D miniaturized, self-organizing structures that mimic the architecture and function of human organs. These systems have transformed our ability to study human development, disease, and therapeutic response in vitro with enhanced biological relevance over traditional 2D culture models.

iPSCs provide an unlimited and ethically sourced starting material to generate diverse organoid systems, including brain, intestine, liver, kidney, and retina. Organoids capture tissue-specific cell diversity and microenvironmental cues, making them valuable tools for disease modeling, drug screening, personalized medicine, and regenerative applications.

Developing robust and reproducible organoids requires precise control over iPSC maintenance, differentiation, and 3D culture conditions. Variability in culture reagents and lack of standardization are common issues that compromise reproducibility and scalability.

Defined Bioscience offers a portfolio of chemically defined, serum-free reagents that support consistent and scalable generation of iPSC-derived organoids. HiDef-B8 provides a robust foundation for feeder-free iPSC culture, while Ready-CEPT ensures high survival during dissociation and plating. Additional supplements like HiDef-N2 and HiDef-ITS promote neural and epithelial lineage specification, respectively. For researchers scaling stem cell production, HiDef-S8 is available in early access to enable high-density suspension-based workflows.

Defined Bioscience
HiDef® B8 stem cell growth medium bottle – defined, serum-free PSC and iPSC media.

HiDef® B8 Stem Cell Growth Medium

From $150.00
From $150.00 $150.00
Defined Bioscience
Ready-CEPT™ cell viability enhancer vial – ROCK inhibitor–based supplement for PSC and iPSC survival.

Ready-CEPT® Cell Viability Enhancer

From $150.00
From $150.00
Defined Bioscience
HiDef® PBS/EDTA cell dissociation reagent bottle – serum-free solution for PSC and iPSC detachment.

HiDef® PBS/EDTA Cell Dissociation Reagent

From $18.00
From $18.00
Defined Bioscience
HiDef® N-2 neural medium supplement bottle – defined, serum-free solution for PSC and iPSC differentiation.

HiDef® N-2 Neural Medium Supplement

From $100.00
From $100.00
Defined Bioscience
HiDef® ITS cell culture supplement bottle – serum-free insulin-transferrin-selenium FBS replacement.

HiDef® ITS Cell Culture Supplement

From $70.00
From $70.00

Key Defined Bioscience Products for Organoid Technology

HiDef-B8: Robust, defined medium for feeder-free iPSC culture.

Ready-CEPT: Cocktail that enhances cell survival during critical transitions like single-cell seeding and organoid initiation.

HiDef PBS/EDTA: Defined phosphate-buffered saline solution for gentle cell dissociation.

HiDef N-2: Supports neural and forebrain organoid generation.

HiDef-ITS: Defined insulin-transferrin-selenium supplement to enhance proliferation efficiency and cell viability.

Application Notes

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High-density pluripotent stem cell expansion in a single-use bioreactor using HiDef S8

High-density pluripotent stem cell expansion in a single-use bioreactor using HiDef S8

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Optimized Expansion, Cryopreservation, and Recovery of Clinical Grade, PluriBank iPSCs Using a Defined Reagent System Kit

Optimized Expansion, Cryopreservation, and Recovery of Clinical Grade, PluriBank iPSCs Using a Defined Reagent System Kit

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Comparative Analysis of 2D and 3D Differentiation Approaches for iPSC-Derived Neural Cells

Comparative Analysis of 2D and 3D Differentiation Approaches for iPSC-Derived Neural Cells

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Isolating Induced Pluripotent Stem Cells by Using the WOLF G2 to Sort Stemness-Related Surface Markers

Isolating Induced Pluripotent Stem Cells by Using the WOLF G2 to Sort Stemness-Related Surface Markers

Read More

Contact and Collaboration Opportunities

Defined Bioscience actively engages with the scientific community to support disease modeling research. We encourage researchers to reach out to our technical experts for collaboration opportunities, protocol optimization, and troubleshooting assistance.

info@definedbioscience.com

By leveraging Defined Bioscience products, researchers can accelerate their disease modeling studies, enhance experimental reliability, and advance therapeutic discoveries.

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