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Defined Bioscience

Developmental Biology

Developmental Biology Using Human Pluripotent STEM Cells

Human induced pluripotent stem cells (iPSCs) are a powerful platform for modeling early human development. Their ability to differentiate into all three germ layers makes them an indispensable tool in studying lineage specification, tissue patterning, and organogenesis under defined and controllable conditions.

iPSCs enable researchers to recapitulate developmental processes in vitro, offering insights into early embryogenesis, cell fate decisions, and congenital disease mechanisms. These models are particularly valuable for studying stages of human development that are otherwise inaccessible due to ethical and practical limitations.

Reproducing the complex and temporally dynamic environment of human development in vitro is difficult. Standardizing culture conditions, achieving precise temporal control of signaling cues, and minimizing variability across experiments remain significant hurdles.

Defined Bioscience offers a suite of fully defined, serum-free reagents that enable precise control over stem cell fate. HiDef-B8 ensures stable and reproducible iPSC maintenance, while Ready-CEPT supports high-efficiency passaging and survival. Supplements like HiDef-N2 and HiDef-ITS provide essential components for guiding neural, mesodermal, and endodermal lineage decisions. HiDef-S8, available via early access, supports high-density cultures for studies requiring large-scale differentiation and sampling.

Defined Bioscience
HiDef-B8 400X Supplement - Defined Bioscience

HiDef® B8 Stem Cell Growth Medium

From $150.00
From $150.00 $150.00
Defined Bioscience
Ready-CEPT™ Cell Viability Enhancer - Defined Bioscience

Ready-CEPT™ Cell Viability Enhancer

From $150.00
From $150.00
Defined Bioscience
HiDef® PBS/EDTA Cell Dissociation Reagent - Defined Bioscience

HiDef® PBS/EDTA Cell Dissociation Reagent

From $18.00
From $18.00
Defined Bioscience
HiDef® N-2 Neural Medium Supplement - Defined Bioscience

HiDef® N-2 Neural Medium Supplement

From $100.00
From $100.00
Defined Bioscience
HiDef-ITS Cell Culture Supplement - Defined Bioscience

HiDef® ITS Cell Culture Supplement

From $70.00
From $70.00

Key Defined Bioscience Products for Developmental Biology

HiDef-B8: Complete, serum-free, defined media ideal for maintaining pluripotency and efficient differentiation.

Ready-CEPT: Optimized supplement promoting enhanced viability and differentiation capability in iPSC-derived cell types.

HiDef PBS/EDTA: Defined phosphate-buffered saline solution for gentle cell dissociation.

HiDef N-2: Defined neural supplement to support robust neural differentiation.

HiDef-ITS: Defined insulin-transferrin-selenium supplement to enhance proliferation efficiency and cell viability.

Application Notes

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High-density pluripotent stem cell expansion in a single-use bioreactor using HiDef S8

High-density pluripotent stem cell expansion in a single-use bioreactor using HiDef S8

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Optimized Expansion, Cryopreservation, and Recovery of Clinical Grade, PluriBank iPSCs Using a Defined Reagent System Kit

Optimized Expansion, Cryopreservation, and Recovery of Clinical Grade, PluriBank iPSCs Using a Defined Reagent System Kit

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Comparative Analysis of 2D and 3D Differentiation Approaches for iPSC-Derived Neural Cells

Comparative Analysis of 2D and 3D Differentiation Approaches for iPSC-Derived Neural Cells

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Isolating Induced Pluripotent Stem Cells by Using the WOLF G2 to Sort Stemness-Related Surface Markers

Isolating Induced Pluripotent Stem Cells by Using the WOLF G2 to Sort Stemness-Related Surface Markers

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Contact and Collaboration Opportunities

Defined Bioscience actively engages with the scientific community to support disease modeling research. We encourage researchers to reach out to our technical experts for collaboration opportunities, protocol optimization, and troubleshooting assistance.

info@definedbioscience.com

By leveraging Defined Bioscience products, researchers can accelerate their disease modeling studies, enhance experimental reliability, and advance therapeutic discoveries.

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