FGF2-G3 (bovine) growth factor

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Biological Activity

btFGF2-G3 has an expected EC50 of less than 1 ng/mL, as determined in-house for all lots by an NIH-3T3 cell proliferation assay.

Key Characteristics

btFGF2-G3 differs from the native sequence of bovine FGF2 (UniProt accession P03969) at nine key residues found to promote increased thermostability and function in cell culture for human FGF2. FGF2-G3 is critical for use in cell culture conditions greater than room temperature; and helps to eliminate the need for weekend supplementation and daily medium changes for stem cell culture.

General Use

btFGF2-G3 is supplied as a 1 mg/mL solution in storage buffer or in a freeze-dried form that can be reconstituted into most standard buffer formulations as needed for downstream use. btFGF2-G3 can be substituted directly for native FGF2, with dosage testing recommended for optimal performance. It is recommended to reconstitute powder btFGF2-G3 to 1 mg/mL with water before use.

References

1- D. Pavel, D. Bednar, P. Vanacek, L. Balek, L. Eiselleova, V. Stepankova, E. Sebestova, M. Kunova Bosakova,
Z. Konecna, S. Mazurenko. A. Kunka, T. Vanova, K. Zoufalova, R. Chaloupkova, J. Brezovsky, P. Krejci, Z.
Prokop, P. Dvorak, J. Damborsky, Biotechnology and Bioengineering 2018, 115, 850.

When is the bovine version used? Ideal for species-specific work such as cultivated meat.

Does it share stability benefits? Yes, identical thermostability improvements as human FGF2-G3.

Is it animal-origin free? Yes, via recombinant E. coli expression.