Defined Bioscience’s HiDef-B8 is a complete, serum-free, defined formulation designed for the feeder-free maintenance and expansion of human pluripotent stem cells (PSCs) in an undifferentiated state.
HiDef-B8 is provided in two formats to offer flexibility for use:
The HiDef-B8 complete medium kit, including the 400X Supplement vial and our DMEM/F12 basal medium;
The HiDef-B8 400X Supplement only (basal medium NOT included), for the modern convenience of basal medium choice.
Defined Bioscience’s HiDef-B8 is a complete, serum-free, defined formulation designed for the feeder-free maintenance and expansion of human pluripotent stem cells (PSCs) in an undifferentiated state. The HiDef-B8 medium is a unique formulation that offers the modern conveniences of a flexible feeding schedule (including weekend-free maintenance) and the ability to choose the matrices and passaging methods that best suit specific applications. HiDef-B8 contains insulin, ascorbic acid-2-phosphate, transferrin, sodium selenite, TGFB3, NRG1, and thermostable FGF2/bFGF. The addition of further components is not required.
Defined Bioscience recommends the use of our DMEM/F12 (catalog # LSB-101) when preparing HiDef-B8 complete medium, as its composition is optimized specifically for HiDef-B8 use. HiDef-B8 400X Supplement compatibility has also been verified with several manufacturers’ formulations of DMEM/F12, maintaining human pluripotent stem cells on matrices from several manufacturers at coating concentrations as low as 2.4 µg per sq-cm (e.g. ~1:800 dilution of 18 mg/mL stock matrix at 1 mL per well in 6-well TC-treated plates), in addition to defined substrates.
This product is shipped on dry ice and should be stored at -20C to -80C until ready for use.