Name: HiDef® B8 Stem Cell Growth Medium
Brand: DefinedBioscience
SKU: LSS-204-1
Price: 150.00 USD
Availability: InStock

$150.00

Shipping calculated at checkout.

Compliance

Research

cGMP

Size

Single

6-pack

12-pack

Format

Supplement (no basal)

Complete (basal & supplement)

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Defined Bioscience’s HiDef® B8 is a complete, serum-free, defined formulation designed for the feeder-free maintenance and expansion of human pluripotent stem cells (PSCs) in an undifferentiated state.

HiDef-B8 is provided in two formats to offer flexibility for use:

The HiDef-B8 complete medium kit, including the 400X Supplement vial and our DMEM/F12 basal medium;
The HiDef-B8 400X Supplement only (basal medium NOT included), for the modern convenience of basal medium choice.

MORE DETAILS:

The HiDef-B8 medium is a unique formulation that offers the modern conveniences of a flexible feeding schedule (including weekend-free maintenance) and the ability to choose the matrices and passaging methods that best suit specific applications. HiDef-B8 contains insulin, ascorbic acid-2-phosphate, transferrin, sodium selenite, TGFB3, NRG1, and thermostable FGF2/bFGF. The addition of further components is not required.

Defined Bioscience recommends the use of our DMEM/F12 (catalog # LSB-101) when preparing HiDef-B8 complete medium, as its composition is optimized specifically for HiDef-B8 use. HiDef-B8 400X Supplement compatibility has also been verified with several manufacturers’ formulations of DMEM/F12 such as Corning® (#10-092), Gibco™ (#11330), and GenClone™ (#25-503), maintaining human pluripotent stem cells on matrices from several manufacturers including Matrigel® (Corning® #356231), Geltrex™ (Gibco™ #A1569601), and ACS-3035™ Cell Basement Membrane (ATCC®) at coating concentrations as low as 2.4 µg cm-2 (e.g. ~1:800 dilution of 18 mg/mL stock matrix at 1 mL per well in 6-well TC- treated plates), in addition to defined substrates such as vitronectin (VTN-N, Gibco™ #A14700). This list of complementary components routinely used in PSC culture is for reference and is not an exhaustive list of verified product compatibility. Contact us at info@definedbioscience.com for more information. Each lot of HiDef-B8 400X Supplement is used in combination with basal medium in performance testing in a culture assay using human iPSCs.

This product is shipped on dry ice and should be stored at -20C to -80C until ready for use.