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Defined Bioscience’s HiDef® B8 is a complete, serum-free, defined formulation designed for the feeder-free maintenance and expansion of human pluripotent stem cells (PSCs) in an undifferentiated state. 

HiDef-B8 is provided in two formats to offer flexibility for use:

  • The HiDef-B8 complete medium kit, including the 400X Supplement vial and our DMEM/F12 basal medium;
  • The HiDef-B8 400X Supplement only (basal medium NOT included), for the modern convenience of basal medium choice.
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The HiDef-B8 medium is a unique formulation that offers the modern conveniences of a flexible feeding schedule (including weekend-free maintenance) and the ability to choose the matrices and passaging methods that best suit specific applications. HiDef-B8 contains insulin, ascorbic acid-2-phosphate, transferrin, sodium selenite, TGFB3, NRG1, and thermostable FGF2/bFGF. The addition of further components is not required.

Defined Bioscience recommends the use of our DMEM/F12 (catalog # LSB-101) when preparing HiDef-B8 complete medium, as its composition is optimized specifically for HiDef-B8 use. HiDef-B8 400X Supplement compatibility has also been verified with several manufacturers’ formulations of DMEM/F12 such as Corning® (#10-092), Gibco™ (#11330), and GenClone™ (#25-503), maintaining human pluripotent stem cells on matrices from several manufacturers including Matrigel® (Corning® #356231), Geltrex™ (Gibco™ #A1569601), and ACS-3035™ Cell Basement Membrane (ATCC®) at coating concentrations as low as 2.4 µg cm-2 (e.g. ~1:800 dilution of 18 mg/mL stock matrix at 1 mL per well in 6-well TC- treated plates), in addition to defined substrates such as vitronectin (VTN-N, Gibco™ #A14700). This list of complementary components routinely used in PSC culture is for reference and is not an exhaustive list of verified product compatibility. Contact us at info@definedbioscience.com for more information. Each lot of HiDef-B8 400X Supplement is used in combination with basal medium in performance testing in a culture assay using human iPSCs.

Defined Bioscience
Defined, serum-free formulation for reliable hiPSC maintenance

HiDef® B8 – Defined Stem Cell Medium for Reliable, Weekend-Free Culture

HiDef® B8 is an optimized, animal-free medium for feeder-free culture of human pluripotent stem cells. It supports flexible, weekend-free schedules and maintains key pluripotency markers over long-term passages — ideal for stem cell and organoid research.

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Application Notes

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Defined Inputs. Consistent Outcomes
Defined Bioscience
Defined Bioscience
Defined Bioscience

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