DMEM/F12 with L-alanyl-L-glutamine and HEPES for improved stability for cell culture
Defined Bioscience’s DMEM/F12 is a fully defined basal medium for use in the culture of a wide range of mammalian cells. DMEM/F12 is a 1:1 mixture of DMEM and Ham’s F-12, providing an optimized environment for a broad range of cell types. Our DMEM/F12 is buffered with HEPES and sodium bicarbonate, offering a comprehensive buffering system which helps to maintain pH stability and minimize the effect of CO2 fluctuations in standard incubator conditions for mammalian cell culture. Additionally, this formulation includes L-alanyl-L-glutamine in place of glutamine, which enhances cell metabolism and reduces the risk of cell death, while also improving medium stability and cell culture consistency.
DMEM/F12 is produced under Current Good Manufacturing Practice (cGMP) regulations, and passes USP standards for sterility, endotoxin (<0.25 EU/mL), and mycoplasma. Product pH is specified at pH 7.2 +/- 0.2 at 20-25°C, and osmolality at 335 +/- 30 mOsm/kg H2O. Please see the CoA for the lot number associated with your order for more information. Our DMEM/F12 includes sodium bicarbonate, HEPES, pyruvate, L alanyl-L-glutamine, and phenol red. Please contact us for a full formulation list.