Cell Dissociation Reagent
HiDef PBS/EDTA
Protein-Free Cell Detachment

HiDef PBS/EDTA Cell Dissociation Reagent

LSR-410-125-1

$18.00
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Defined Bioscience’s HiDef PBS/EDTA is a defined reagent for protein-free adherent cell dissociation, for routine aggregate passaging or single cell suspension. HiDef PBS/EDTA is designed to detach cells from surfaces without impacting cell functionality or surface protein integrity, with applications in stem, tumor, and primary cell culture, among others. HiDef PBS/EDTA can also be used as a cell washing step prior to detachment using other methods, including enzymatic digestion (e.g. trypsinization).

HiDef PBS/EDTA is provided as a 1x solution, 125 mL per bottle, and consists of Dulbecco’s Phosphate Buffered Saline (DPBS, without Ca2+ and Mg2+) pH 7.4 with 0.5 mM EDTA. Each lot of HiDef PBS/EDTA is used in combination with HiDef-B8 medium in performance testing in a culture assay evaluating using human iPSCs to confirm appropriate and consistent detachment.

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HiDef PBS/EDTA Cell Dissociation Reagent - Defined Bioscience

HiDef PBS/EDTA for protein-free adherent cell dissociation

Defined Bioscience’s HiDef PBS/EDTA is a defined reagent for protein-free adherent cell dissociation, for routine aggregate passaging or single cell suspension. HiDef PBS/EDTA is designed to detach cells from surfaces without impacting cell functionality or surface protein integrity, with applications in stem, tumor, and primary cell culture, among others. HiDef PBS/EDTA can also be used as a cell washing step prior to detachment using other methods, including enzymatic digestion (e.g. trypsinization).

HiDef PBS/EDTA is provided as a 1x solution, 125 mL per bottle, and consists of Dulbecco’s Phosphate Buffered Saline (DPBS, without Ca2+ and Mg2+) pH 7.4 with 0.5 mM EDTA. Each lot of HiDef PBS/EDTA is used in combination with HiDef-B8 medium in performance testing in a culture assay evaluating using human iPSCs to confirm appropriate and consistent detachment.

Product Specifications

HiDef PBS/EDTA is provided at 1x in liquid form in 125-mL volumes. This supplement can be used with cells cultured in a wide range of medium formulations, including Defined Bioscience’s fully defined HiDef-B8 stem cell medium (Catalog #LSS-204).

Each lot of HiDef PBS/EDTA is used in performance testing in a culture assay evaluating cell detachment.