Defined Bioscience’s HiDef PBS/EDTA is a defined reagent for protein-free adherent cell dissociation, for routine aggregate passaging or single cell suspension. HiDef PBS/EDTA is designed to detach cells from surfaces without impacting cell functionality or surface protein integrity, with applications in stem, tumor, and primary cell culture, among others. HiDef PBS/EDTA can also be used as a cell washing step prior to detachment using other methods, including enzymatic digestion (e.g. trypsinization).

HiDef PBS/EDTA is provided as a 1x solution, 125 mL per bottle, and consists of Dulbecco’s Phosphate Buffered Saline (DPBS, without Ca2+ and Mg2+) pH 7.4 with 0.5 mM EDTA. Each lot of HiDef PBS/EDTA is used in combination with HiDef-B8 medium in performance testing in a culture assay evaluating using human iPSCs to confirm appropriate and consistent detachment.