FGF2 variants for life science and cultivated meat
Naturally occurring isoforms of growth factors offer a range of unique features across diverse applications, and suggest means for evolution-driven improvements in stability, function, and efficacy. We produced a range of FGF2 homologs, coupled with the structure determination of thermostable human variant FGF2-G3, to inform the development of new growth factor solutions. Presented at ISSCR 2022.
Engineering approaches to medium optimization
Current growth medium solutions for the cell culture space are oftentimes inconsistent, variable, and costly, warranting the development of more optimized alternatives. We applied a design of experiments (DOE) to fit a response surface methodology (RSM) model to HiDef-B8 medium for continued optimization in stem cell culture. Presented at ISSCR 2022.
FGF2-G3 usage in HiDef-B8
Serum-containing media present major obstacles for the cell culture space, including batch-to-batch variability, undefined componentry, and the introduction of growth-inhibiting factors. We produced FGF2-G3, a thermostable variant of human FGF2, and studied its use in the context of HiDef-B8, a serum-free, fully-defined formulation for the culture of stem cells. Presented at ISSCR 2022.