App Notes & Posters

Growth factor production for enhanced growth of fish multipotent stem cells for cell-cultivated meat

Varied growth factor performance across homologs

Growth factor homologs across species can exhibit naturally evolved differences in function and in vitro stability. Continuing work previously presented at ISSCR, we studied changes in thermostability of recombinantly produced FGF2 homologs in advance of cell culture efforts. This work highlighted the value of variations in nature that can contribute to growth factor performance. Presented at ISSCR 2024 - Cincinnati Symposium.

FGF2, part two: evaluation of homologs for use in human and animal cell culture for the life sciences and cultivated meat

FGF2 homologs in the context of cell culture

Sequence homologs of Basic Fibroblast Growth Factor (bFGF or FGF2) have exhibited differential performance in cell growth across species, with clear implications for improving cost and performance of human- and animal-derived cell culture. Continuing from work presented at ISSCR 2022 (see previous poster below), we evaluated several candidates for proliferation of human- and animal-derived cell lines. These results highlight the value of growth factor homolog screening when optimizing cell culture conditions. Presented at ISSCR 2023.

Defined cGMP animal-origin-free medium supports PSC during, and promotes single-cell clonal expansion after, microfluidic sorting

HiDef-B8 for single-cell clonal expansion

Fully defined and animal/serum-free media, manufactured under strict cGMP protocols and with traceable sourcing, are of high value in pluripotent stem cell isolation and expansion. Continuing from our collaboration with NanoCellect Biomedical (see application note below), we explored the value of our such medium, HiDef-B8, in the culture of hiPSCs. Presented at ISSCR 2023.

Optimized HiDef-B8 Reduces Costs and Improves Accessibility for PSC Research

HiDef-B8 optimization using DOE

Consistency in growth media remains a challenge for stem cell research and manufacturing. Continuing from our work presented at ISSCR 2022 (see poster below), we continued our validation of HiDef-B8 using DOE-driven approaches, focusing on three components of the medium. Of the variants tested, HiDef-B8 continued to outperform based on function and cost. Presented at ISSCR 2023.

Isolating Induced Pluripotent Stem Cells by Using the WOLF G2 to Sort Stemness-Related Surface Markers

Gentle sorting of hiPSCs grown in HiDef-B8 medium

Gentle cell sorting technologies are advantageous in the isolation and expansion of homogeneous hiPSCs, as is the use of fully defined and animal-free cell media. In collaboration with NanoCellect Biomedical, we grew hiPSCs in HiDef-B8 and used the WOLF G2 to identify and enrich for hiPSCs expressing SSEA-4 and TRA-1-60-R. HiDef-B8 grown hiPSCs showed healthy morphology, viability, and expression of these markers, with robust post-sorting recovery in the same medium.

Development of FGF2 homologs for applications in human stem cell culture and the cultivated meat industry

FGF2 variants for life science and cultivated meat

Naturally occurring isoforms of growth factors offer a range of unique features across diverse applications, and suggest means for evolution-driven improvements in stability, function, and efficacy. We produced a range of FGF2 homologs, coupled with the structure determination of thermostable human variant FGF2-G3, to inform the development of new growth factor solutions. Presented at ISSCR 2022.

Defining Culture: Cost-effective Medium to Improve Stem Cell Reproducibility

Engineering approaches to medium optimization

Current growth medium solutions for the cell culture space are oftentimes inconsistent, variable, and costly, warranting the development of more optimized alternatives. We applied a design of experiments (DOE) to fit a response surface methodology (RSM) model to HiDef-B8 medium for continued optimization in stem cell culture. Presented at ISSCR 2022.

Growth factor production and optimization for fully-defined media in induced pluripotent stem cell culture

FGF2-G3 usage in HiDef-B8

Serum-containing media present major obstacles for the cell culture space, including batch-to-batch variability, undefined componentry, and the introduction of growth-inhibiting factors. We produced FGF2-G3, a thermostable variant of human FGF2, and studied its use in the context of HiDef-B8, a serum-free, fully-defined formulation for the culture of stem cells. Presented at ISSCR 2022.