Thermostable
FGF2-G3
Engineered to last weeks
(not hours)
FGF2-G3, thermostable, hyperstable FGF2

FGF2-G3 Thermostable Growth Factor Cat # LSR-101

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FGF2-G3 (FGF2-STAB) is a stabilized growth factor that offers a novel way to grow FGF2-dependent cell cultures more efficiently, with fewer media changes. FGF2-G3 retains full biological activity even after five days at 37°C. The stable level of FGF2 in culture allows for a more homogenous, undifferentiated stem cell culture, while saving researchers valuable time and money, as repeated supplementation by FGF2 and every day medium change is not required. Contact us for larger quantities to get a quote.

Introduction

FGF2-G3 is a stabilized growth factor that offers a novel way to grow FGF2-dependent cell cultures more efficiently, with fewer media changes. FGF2-G3 retains full biological activity even after twenty days at 37°C. The stable level of FGF2 in culture allows for a more homogenous, undifferentiated stem cell culture, while saving researchers valuable time and money because repeated supplementation of FGF2 and a daily medium change is not required. FGF2 is a non-glycosylated heparin binding growth factor that is expressed in the brain, pituitary gland, kidney, retina, bone, testis, adrenal gland, liver, monocytes, epithelial cells and endothelial cells. FGF2 functions as a pleiotropic regulator of proliferation, differentiation, migration, and survival in a variety of cell types and is an essential component of media for the cultivation of human pluripotent stem cells because it helps maintain the cells in the pluripotent state.

FGF2-G3 has nine key mutations that were chosen through iterative trials of in silico modeling and empirical testing, improved the half-life of human FGF2 from 10-12 hours at 37°C to over three weeks. FGF2-G3 was used in the B8 formulation detailed in recent publications from Northwestern University for the maintenance of human iPS cells, where it could be used to the same effect as wild-type FGF2 but with lower concentrations. The medium itself can also be used in a weekend-free format as well, likely due to the high thermostability of FGF2-G3. The high-efficacy and thermostability of this factor, combined with its animal-free status with production in bacteria, make it a promising ingredient for the culture of stem cells in the life science, pharmaceutical, and cultivated meat industries.

Improved biological half-life of FGF2-G3 compared to previous iterations (G2) and the wild-type sequence (G0) by densitometric analysis of ERK1/2 Western blotting.

Type: Recombinant human protein, E. coli-derived, His-tagged
Purity: >95% by SDS PAGE under reducing conditions
Endotoxin: Endotoxin level is <0.1 ng/μg of protein (<1 EU/μg)
Storage & Stability: Upon arrival store at -20°C to -80°C. FGF2-G3 is stable for up to 12 months when stored at -20°C to -80°C.
Intended use: For research use only