DMEM/F12 Basal Medium

Defined Bioscience’s DMEM/F12 is a fully defined basal medium for use in the culture of a wide range of mammalian cells. DMEM/F12 is a 1:1 mixture of DMEM and Ham’s F-12, buffered with HEPES and sodium bicarbonate for more comprehensive buffering, and substituted with L-alanyl-L-glutamine in place of glutamine for improved cell health and product stability. DMEM/F12 should be used with other growth factors, sera, or additives (NOT INCLUDED) depending on your cell culture application.

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Defined Bioscience’s DMEM/F12 is a fully defined basal medium for use in the culture of a wide range of mammalian cells. DMEM/F12 is a 1:1 mixture of DMEM (a modified Basal Medium Eagle (BME) formulation with higher concentrations of amino acids and vitamins) and Ham’s F-12 (modified from Ham’s F-10 with increased choline, inositol, putrescine, and other amino acids), providing an optimized environment for a broad range of cell types. The medium is designed to improve cell growth and maintain cell health, resulting in improved cell viability and enhanced metabolic activity. Our DMEM/F12 is buffered with HEPES and sodium bicarbonate, offering a comprehensive buffering system which helps to maintain pH stability and minimize the effect of CO2 fluctuations in standard incubator conditions for mammalian cell culture. Additionally, this formulation includes L-alanyl-L-glutamine in place of glutamine, which enhances cell metabolism and reduces the risk of cell death, while also improving medium stability and cell culture consistency. DMEM/F12 contains no proteins, lipids or growth factors, and depending on your intended use, may require supplementation with growth factors or serum.

With Defined Bioscience's DMEM/F12, you can achieve higher cell densities, improve protein expression, and optimize the performance of your cells. This medium is ideal for a wide range of cell culture applications, including the culture of primary cells, stem cells, and various cell lines. The medium is sterile and free from mycoplasma contamination, and it is compatible with standard culture conditions, including CO2 incubation. With its unique blend of DMEM and Ham's F-12 media, HEPES-based buffering, and L-alanyl-L-glutamine, our DMEM/F12 provides a reliable, consistent, and high-performance cell culture medium for your research needs.

Our DMEM/F12 can be used in place of standard DMEM/F12 for most applications, and can also be used in combination with Defined Bioscience’s HiDef-B8 400X Supplement (Catalog # LSS-204) for a complete pluripotent stem cell (PSC) maintenance medium. DMEM/F12 is sold in 500-mL units in PET bottles (with ~130-mL overhead space) with HDPE caps, and in six- or 12-bottle cases.