{"product_id":"hidef-s8-stem-cell-suspension-medium","title":"HiDef® S8 Stem Cell Suspension Medium","description":"\u003cp data-start=\"238\" data-end=\"494\" class=\"PDq2pG_selectionAnchorContainer\"\u003eDefined Bioscience's HiDef® S8 is a complete, serum-free, defined formulation designed for the high-density, microcarrier-free suspension expansion of human pluripotent stem cells (PSCs) in stirred-tank bioreactors and other suspension culture systems.\u003cspan aria-hidden=\"true\" class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp data-start=\"496\" data-end=\"535\"\u003e\u003cstrong data-start=\"496\" data-end=\"535\"\u003eHiDef® S8 is currently provided as:\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul data-start=\"537\" data-end=\"745\"\u003e\n\u003cli data-section-id=\"10zwweq\" data-start=\"537\" data-end=\"745\"\u003eThe HiDef® S8 400X Supplement only (DMEM\/F12 basal medium and optional surfactant NOT included), allowing users the flexibility to select their preferred basal medium and suspension workflow components.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp data-start=\"747\" data-end=\"1100\"\u003eTo better support small-scale experiments and pilot studies, the HiDef® S8 400X Supplement is offered in two vial sizes. Standard vials prepare 500 mL of complete medium, while miniature vials prepare 50 mL, enabling convenient preparation of smaller volumes without excess waste. See instructions below regarding preparation of each format.\u003c\/p\u003e\n\u003ch3 data-section-id=\"hn7j01\" data-start=\"1102\" data-end=\"1118\"\u003eMore Details\u003c\/h3\u003e\n\u003cp data-start=\"1120\" data-end=\"1744\"\u003eHiDef® S8 is a unique formulation that enables high-yield, scalable suspension expansion of human pluripotent stem cells without the need for microcarriers. The medium supports efficient aggregate formation from single-cell or small aggregate inoculation, rapid biomass accumulation, and expansion to tens of millions of PSCs per milliliter while maintaining high viability, uniform aggregate morphology, stable size distribution, and expression of key pluripotency markers. Following suspension expansion, cells readily transition back to adherent culture while preserving normal morphology and pluripotent characteristics.\u003c\/p\u003e\n\u003cp data-start=\"1746\" data-end=\"2167\"\u003eHiDef® S8 contains a combination of essential nutrients and growth factors formulated specifically to maintain pluripotency while supporting robust suspension growth under agitation conditions. The addition of further components is not required, although optional surfactants such as polyvinyl alcohol (PVA), Pluronic® F-68, dextran sulfate, and others may be incorporated where desired for specific suspension workflows.\u003c\/p\u003e\n\u003cp data-start=\"2169\" data-end=\"2781\"\u003eHiDef® S8 400X Supplement has been verified for compatibility with standard formulations of DMEM\/F12 from several manufacturers, including Corning® (#10-092), Gibco™ (#11330), and GenClone™ (#25-503). Optional surfactants including 0.1% (w\/v) polyvinyl alcohol (PVA), 0.1% (w\/v) Pluronic® F-68, dextran sulfate, and related additives may be used depending on the application and bioreactor configuration. This list is provided for reference and is not an exhaustive list of verified compatible components. Contact us at \u003ca data-start=\"2711\" data-end=\"2737\" class=\"decorated-link cursor-pointer\" rel=\"noopener\"\u003einfo@definedbioscience.com\u003c\/a\u003e for additional compatibility information.\u003c\/p\u003e\n\u003cp data-start=\"2783\" data-end=\"3046\" data-is-last-node=\"\" data-is-only-node=\"\"\u003eEach lot of HiDef® S8 400X Supplement is used in combination with basal medium during performance qualification using multiple human PSC lines cultured under suspension conditions in both ambient and hypoxic environments.\u003c\/p\u003e","brand":"DefinedBioscience","offers":[{"title":"Single","offer_id":50530367897823,"sku":null,"price":225.0,"currency_code":"USD","in_stock":true},{"title":"6-pack","offer_id":50530367963359,"sku":null,"price":1305.0,"currency_code":"USD","in_stock":true},{"title":"12-pack","offer_id":50530368028895,"sku":null,"price":2520.0,"currency_code":"USD","in_stock":true},{"title":"Minis","offer_id":50530368094431,"sku":null,"price":275.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0661\/1812\/3743\/files\/S8_nolabel_1.png?v=1783348294","url":"https:\/\/definedbioscience.com\/products\/hidef-s8-stem-cell-suspension-medium","provider":"Defined Bioscience","version":"1.0","type":"link"}